Mar 23, 2023
This podcast covers several common separation and purification laboratory techniques that you may see on the MCAT. I have broken this subject down into three different categories: small molecules, proteins, and cells. Within the small molecule category, I cover: extraction, crystallization and recrystallization, and chromatography (TLC and HPLC). In the protein category, I cover: SDS-page, precipitation, isoelectric focusing, and chromatography (size-exclusion, affinity, and ion-exchange). Last, in the cell category, I briefly cover: centrifugation, flow cytometry, and microfluidics.
Please email me if you have any comments or concerns: MCATpodcast@medschoolcoach.com
Thanks for listening!
To learn more about how MedSchoolCoach can help you along your medical school journey, visit us at Prospective Doctor.
CORRECTION: When talking about liquid-liquid extractions, Sam says that when the pH of a solution is below the pKa of the solution it will be deprotonated and be negatively charged. This is incorrect. When the pH of a solution is below its pKa, it would be protonated and potentially positively charged.