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MCAT Basics (from MedSchoolCoach)

Mar 23, 2023

This podcast covers several common separation and purification laboratory techniques that you may see on the MCAT. I have broken this subject down into three different categories: small molecules, proteins, and cells. Within the small molecule category, I cover: extraction, crystallization and recrystallization, and chromatography (TLC and HPLC). In the protein category, I cover: SDS-page, precipitation, isoelectric focusing, and chromatography (size-exclusion, affinity, and ion-exchange). Last, in the cell category, I briefly cover: centrifugation, flow cytometry, and microfluidics.

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CORRECTION: When talking about liquid-liquid extractions, Sam says that when the pH of a solution is below the pKa of the solution it will be deprotonated and be negatively charged. This is incorrect. When the pH of a solution is below its pKa, it would be protonated and potentially positively charged.